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TP 5000:Lieber-DeCarli 71%高脂模型饲料
Lieber-DeCarli Diet with 71% High Fat

饲料代码TP 5000是Lieber-DeCarli液体型71%高脂模型饲料,适用于大鼠和小鼠。

Lieber和DeCarli首先研究出36%高脂酒精液体饲料建立酒精性脂肪肝模型(AFLD)饲料,由于该饲料是至今仍广为使用,这就是经典而闻名的Lieber-DeCarli酒精液体饲料。该36%酒精液体饲料的对照饲料是36%高脂饲料,也就是说,模型饲料和对照饲料都是36%高脂肪,不同的是模型饲料中有酒精,对照饲料没有酒精。

Lieber-DeCarli 71%高脂模型饲料的特点

(1)Lieber等作者的研究表明,用于建立非酒精性脂肪肝的速度非常快,只需要几周。脂肪肝为全小叶性,伴有单核细胞浸润(炎症),线粒体损伤,氧化应激,肿瘤坏死因子升高,I型胶原纤维和前胶原mRNA增加,胰岛素升高。

(2)饲料为液体型。研究者可以自行向液体饲料中任意添加药物、食物提取物或其他功能因子。这是固体型饲料无法做到的。

(3)改变脂肪类型,可以研究不同脂肪(不同脂肪酸构成)对NSAH(NAFLD)形成和干预的作用。

后来,Lieber和DeCarli又转向研究建立非酒精性脂肪肝模型(NAFLD, NASH)模型,方法是在对照饲料36%高脂基础上将脂肪热量提高到71%,碳水化合物热量相应减少到11%,蛋白质热量不改变(18%),并且仍使用液体型饲料喂养。这个模型饲料喂养SD大鼠后,非酒精性脂肪肝发展很快,几周就非常明显,不仅肝脏病理表现与人类相似,而且伴有高胰岛素血症(胰岛素抵抗)、肿瘤坏死因子(TNF)等一系列生化指标的改变。

奇怪的是,后来国外有一篇SCI论文,质疑该模型饲料能否引起非酒精性脂肪肝。

然而,后来有些研究者使用该饲料都成功复制出来了非酒精性脂肪肝模型,并且病理表现和生化指标的改变都得到重复和验证。有的研究者在基因敲除的小鼠中也建模成功。

南通特洛菲饲料科技有限公司按照作者提供的信息进行科学配比,饲料代码TP5000,对照饲料是TP 4030C,或标准对照TP4010、TP4012。供广大研究者进行非酒精性脂肪肝的研究。

液体饲料喂养瓶

用于液体饲料的喂养瓶


瓶子固定容易、牢靠。

瓶体透明、有刻度,可以估计摄食量,通过称量可以精确得到消耗量。

饮液管不漏、不堵。

价格低廉,用户评价高。

 

 

References:

[1] Wang Y, Ausman LM, Russell RM, Greenberg AS, Wang X. Increased apoptosis in high-fat diet-induced nonalcoholic steatohepatitis in rats is associated with c-Jun NH2-terminal kinase activation and elevated proapoptotic Bax. D. J Nutr. 2008 Oct;138(10):1866-71.

[2] Akin H, Deniz M, Tahan V, Can G, Kedrah AE, Celikel C, T"ozün N, Imeryüz N. High-fat liquid "Lieber-DeCarli" diet for an animal model of nonalcoholic steatohepatitis: does it really work? Hepatol Int. 2007 Dec;1(4):449-50.

[3] Wang Y, Ausman LM, Greenberg AS, Russell RM, Wang XD. Nonalcoholic steatohepatitis induced by a high-fat diet promotes diethylnitrosamine-initiated early hepatocarcinogenesis in rats. Int J Cancer. 2009 Feb 1;124(3):540-6.

[4] Wang Y, Ausman LM, Greenberg AS, Russell RM, Wang XD. Dietary lycopene and tomato extract supplementations inhibit nonalcoholic steatohepatitis-promoted hepatocarcinogenesis in rats. Int J Cancer. 2010 Apr 15;126(8):1788-96.

[5] Ahmed U, Redgrave TG, Oates PS. Effect of dietary fat to produce non-alcoholic fatty liver in the rat. J Gastroenterol Hepatol. 2009 Aug;24(8):1463-71.

[6] Takahashi Y, Soejima Y, Fukusato T. Animal models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis. World J Gastroenterol. 2012 May 21;18(19):2300-8.

[7] Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, Ponomarenko A, DeCarli LM. Model of nonalcoholic steatohepatitis. Am J Clin Nutr. 2004;79:502–9.

[8] Melendez-Martinez AJ, Nascimento AF, Wang Y, Liu C, Mao Y, Wang XD. Effect of tomato extract supplementation against high-fat diet-induced hepatic lesions.Hepatobiliary Surg Nutr. 2013 Aug;2(4):198-208.

[9] Ahmed U, Redgrave TG, Oates PS. Effect of dietary fat to produce non-alcoholic fatty liver in the rat.J Gastroenterol Hepatol. 2009 Aug;24(8):1463-71.

[10] Esposito E, Iacono A, Bianco G, Autore G, Cuzzocrea S, Vajro P, Canani RB, Calignano A, Raso GM, Meli R. Probiotics reduce the inflammatory response induced by a high-fat diet in the liver of young rats. J Nutr. 2009 May;139(5):905-11.

[11] Nikolaenko L, Jia Y, Wang C, Diaz-Arjonilla M, Yee JK, French SW, Liu PY, Laurel S, Chong C, Lee K, Lue Y, Lee WN, Swerdloff RS.Testosterone Replacement Ameliorates Non-Alcoholic Fatty Liver Disease in Castrated Rats. Endocrinology. 2013 Nov 26

[12] Haddad Y, Vallerand D, Brault A, Spénard J, Haddad PS. NCX 1000 Alone or in Combination with Vitamin E Reverses Experimental Nonalcoholic Steatohepatitis in the Rat Similarly to UDCA. Int J Hepatol. 2011;2011:136816.

[13] Tervola KM, Gummer MA, Erdman JW Jr, O'Brien WD Jr. Ultrasonic attenuation and velocity properties in rat liver as a function of fat concentration: a study at 100 MHz using a scanning laser acoustic microscope.J Acoust Soc Am. 1985 Jan;77(1):307-13.

[14] Wilkins T, Tadkod A, Hepburn I, Schade RR. Nonalcoholic fatty liver disease: diagnosis and management. Am Fam Physician. 2013 Jul 1;88(1):35-42

[15] Syn WK, Choi SS, Diehl AM. Apoptosis and cytokines in non-alcoholic steatohepatitis.Clin Liver Dis. 2009 Nov;13(4):

[16] Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, Ponomarenko A, DeCarli LM. Acarbose attenuates experimental non-alcoholic steatohepatitis. Biochem Biophys Res Commun. 2004 Mar 12;315(3):699-703.

[17] Romestaing C, Piquet MA, Bedu E, Rouleau V, Dautresme M, Hourmand-Ollivier I, Filippi C, Duchamp C, Sibille B. Long term highly saturated fat diet does not induce NASH in Wistar rats. Nutr Metab (Lond). 2007 Feb 21;4:4.

[18] Lieber CS, DeCarli LM, Leo MA, Mak KM, Ponomarenko A, Ren C, Wang X. Beneficial effects versus toxicity of medium-chain triacylglycerols in rats with NASH.J Hepatol. 2008 Feb;48(2):318-26.

[19] Abdelmegeed MA, Yoo SH, Henderson LE, Gonzalez FJ, Woodcroft KJ, Song BJ. PPARalpha expression protects male mice from high fat-induced nonalcoholic fatty liver. J Nutr. 2011 Apr 1;141(4):603-10.

[20] Wang Y, Seitz HK, Wang XD. Moderate alcohol consumption aggravates high-fat diet induced steatohepatitis in rats. Alcohol Clin Exp Res. 2010 Mar 1;34(3):567-73.

[21] Eccleston HB1, Andringa KK, Betancourt AM, King AL, Mantena SK, Swain TM, Tinsley HN, Nolte RN, Nagy TR, Abrams GA, Bailey SM. Chronic exposure to a high-fat diet induces hepatic steatosis, impairs nitric oxide bioavailability, and modifies the mitochondrial proteome in mice. Antioxid Redox Signal. 2011 Jul 15;15(2):447-59.



有困惑?那就商量呗!

大鼠小鼠71%高脂造模饲料

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markerTP5000

TP5000是Lieber-DeCarli 71%高脂模型饲料,用于非酒精性脂肪肝模型复制。

markerTP4031

TP4031是TP5000的对照饲料,脂肪热量36%。

markerTP4010,TP4012

TP4010和TP4012用于标准喂养对照,TP4010用于未成年、妊娠、哺乳期,TP4012用于未成年。两者都是液体饲料。



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